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anti tra 1 60  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology anti tra 1 60
    Anti Tra 1 60, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 314 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+tra+1+60/TRA-1-60+Antibody/pm41916852-424-5-8
    Average 94 stars, based on 314 article reviews
    anti tra 1 60 - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Incubation:

    Article Title: Thermostable Human Basic Fibroblast Growth Factor (TS-bFGF) Engineered with a Disulfide Bond Demonstrates Superior Culture Outcomes in Human Pluripotent Stem Cell
    Article Snippet: The cells were permeabilized with 0.5% Triton X-100 in 1× PBS without Ca 2+ and Mg 2+ for 10 min and blocked with 1× PBS with 1% bovine serum albumin (BSA; Bovogen, Australia) for 1 h at RT. .. The cells were incubated with specific primary antibodies, including anti-TRA-1-60 (1:200; Santa Cruz Biotechnology, Dallas, TX, USA, sc-21705) and anti-SSEA4 (1:200; Santa Cruz Biotechnology, sc-21704) at 4 °C overnight. ..

    Article Title: Inhibiting mitochondrial fission rescues degeneration in hereditary spastic paraplegia neurons
    Article Snippet: .. A 0.2% Triton-X solution in PBS was used for permeabilization for 10 min, and 10% donkey serum solution in PBS was used for blocking for 1 h. Cells were incubated with primary antibodies overnight and secondary antibodies for 1 h. Antibodies used included: anti-TRA-1-60 (mouse IgM, Santa Cruz, 1:50); anti-NANOG (goat IgG, R&D Systems, 1:500); anti-SSEA4 (mouse IgG3, DSHB, 1:100); anti-OCT4 (mouse IgG, Santa Cruz, 1:200); anti-CTIP2 (rat IgG, Abcam, 1:2000); anti-NEUN (rabbit IgG, Abcam, 1:500); anti-NF200 (rabbit IgG, Sigma-Aldrich, 1:1000), anti-p-NFH (mouse IgG, Millipore, 1:1000); anti-Tom20 (mouse IgG, Proteintech, 1:500); anti-UCHL1 (Rabbit IgG, Proteintech, 1:500). ..

    Article Title: Thermostable Human Basic Fibroblast Growth Factor (TS-bFGF) Engineered with a Disulfide Bond Demonstrates Superior Culture Outcomes in Human Pluripotent Stem Cell.
    Article Snippet: The cells were permeabilized with 0.5% Triton X-100 in 1× PBS without Ca2+ and Mg2+ for 10 min and blocked with 1× PBS with 1% bovine serum albumin (BSA; Bovogen, Australia) for 1 h at RT. .. The cells were incubated with specific primary antibodies, including anti-TRA-1-60 (1:200; Santa Cruz Biotechnology, Dallas, TX, USA, sc-21705) and anti-SSEA4 (1:200; Santa Cruz Biotechnology, sc-21704) at 4 ◦C overnight. ..

    Control:

    Article Title: Clinical-scale bioreactor production of hiPSC-derived extracellular vesicles modulates miRNA and protein cargo to enhance angiogenic function.
    Article Snippet: A perfusion-based stirred-tank bioreactor enables scalable production of human induced pluripotent stem cell–derived extracellular vesicles (EVs) enriched in proangiogenic cargo.. This approach overcomes key limitations of conventional EV production, delivering high yields and consistent quality, and represents an important step toward standardized, clinically relevant EV manufa cturing for cell-free regenerative therapies.

    Blocking Assay:

    Article Title: Inhibiting mitochondrial fission rescues degeneration in hereditary spastic paraplegia neurons
    Article Snippet: .. A 0.2% Triton-X solution in PBS was used for permeabilization for 10 min, and 10% donkey serum solution in PBS was used for blocking for 1 h. Cells were incubated with primary antibodies overnight and secondary antibodies for 1 h. Antibodies used included: anti-TRA-1-60 (mouse IgM, Santa Cruz, 1:50); anti-NANOG (goat IgG, R&D Systems, 1:500); anti-SSEA4 (mouse IgG3, DSHB, 1:100); anti-OCT4 (mouse IgG, Santa Cruz, 1:200); anti-CTIP2 (rat IgG, Abcam, 1:2000); anti-NEUN (rabbit IgG, Abcam, 1:500); anti-NF200 (rabbit IgG, Sigma-Aldrich, 1:1000), anti-p-NFH (mouse IgG, Millipore, 1:1000); anti-Tom20 (mouse IgG, Proteintech, 1:500); anti-UCHL1 (Rabbit IgG, Proteintech, 1:500). ..

    Article Title: Hair Follicle Organoids Using Human iPSC-Derived Ectodermal Precursor Cells for Hair Regenerative Medicine.
    Article Snippet: .. The samples were treated with blocking solution for 1 h at 25 °C, and further stained with anti-Versican (Merck Millipore), anti-CD34 (Thermo Fisher Scientific, Inc.), anti-Sox2 (Abcam), anti-KRT15 (Abcam), anti-c-KIT (Abcam), anti-gp100 (Abcam), anti-SSEA-4 (Abcam), anti-OCT-3/4 (R&D Systems), anti-TRA-1−60 (Santa Cruz Biotechnology) and anti-KRT18 (Cloud-Clone Corp.) for 2 h at 4 °C, followed by respective secondary antibody staining for 2 h at 25 °C, and finally, 1 μg/mL 4′,6-diamidino-2-phenylindole in PBS for 10 min at 25 °C. .. For ALP sample staining, an ALP-leukocyte kit was used according to the manufacturer’s protocol (Sigma-Aldrich).

    Staining:

    Article Title: Hair Follicle Organoids Using Human iPSC-Derived Ectodermal Precursor Cells for Hair Regenerative Medicine.
    Article Snippet: .. The samples were treated with blocking solution for 1 h at 25 °C, and further stained with anti-Versican (Merck Millipore), anti-CD34 (Thermo Fisher Scientific, Inc.), anti-Sox2 (Abcam), anti-KRT15 (Abcam), anti-c-KIT (Abcam), anti-gp100 (Abcam), anti-SSEA-4 (Abcam), anti-OCT-3/4 (R&D Systems), anti-TRA-1−60 (Santa Cruz Biotechnology) and anti-KRT18 (Cloud-Clone Corp.) for 2 h at 4 °C, followed by respective secondary antibody staining for 2 h at 25 °C, and finally, 1 μg/mL 4′,6-diamidino-2-phenylindole in PBS for 10 min at 25 °C. .. For ALP sample staining, an ALP-leukocyte kit was used according to the manufacturer’s protocol (Sigma-Aldrich).

    other:

    Article Title: Establishment of feline embryonic stem cells from the inner cell mass of blastocysts produced in vitro
    Article Snippet: Anti-Tra-1-60 , Mouse , Sc-21705 , TRA-1-60 , Monoclonal , 1:100 , Santa cruz biotechnology.



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    Reprogramming of adult human dermal fibroblasts (HDFa) into induced pluripotent stem cell (iPSC) line BO-VC1. Reprogramming was performed using the Epi5 ™ Episomal iPSC Reprogramming Kit, enabling the generation of ( A ) transgene- and virus-free iPSC line BO-VC1. Successful generation of fibroblast-derived iPSCs was validated by immunocytochemical staining for the pluripotency markers ( B ) SOX2, C TRA 1–60, D OCT4, SSEA4 and E NANOG. Quantification of the generated iPSCs via flow cytometry revealed F 99.31% <t>SSEA4/TRA1-60,</t> G 97.43% SOX2/TRA1-60 and H 98.60% OCT3/4/TRA1-60 positive cells. I Quantification of the transcript levels of the stem cell markers NANOG , OCT4 , REX1 and SOX2 revealed significantly higher mRNA levels in iPSC line BO-VC1 compared to the HDFa control ( n = 3 different passages) Moreover, generated iPSCs were functionally validated by directed differentiation into all three germ layers. Subsequent immunocytochemical staining of the iPSCs before and after differentiation confirmed the expression of ( J–M ) meso-, N–Q endo- and R–U ectodermal lineage markers only in the respective differentiations. Scale bars: 50 μm. Data were tested for normal distribution using Shapiro-Wilk test. Means ± SEM (standard error of the mean) were statistically analyzed by a Kruskal-Wallis test with Dunn’s multiple comparisons test. n = 3 (* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001)
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    Reprogramming of adult human dermal fibroblasts (HDFa) into induced pluripotent stem cell (iPSC) line BO-VC1. Reprogramming was performed using the Epi5 ™ Episomal iPSC Reprogramming Kit, enabling the generation of ( A ) transgene- and virus-free iPSC line BO-VC1. Successful generation of fibroblast-derived iPSCs was validated by immunocytochemical staining for the pluripotency markers ( B ) SOX2, C TRA 1–60, D OCT4, SSEA4 and E NANOG. Quantification of the generated iPSCs via flow cytometry revealed F 99.31% <t>SSEA4/TRA1-60,</t> G 97.43% SOX2/TRA1-60 and H 98.60% OCT3/4/TRA1-60 positive cells. I Quantification of the transcript levels of the stem cell markers NANOG , OCT4 , REX1 and SOX2 revealed significantly higher mRNA levels in iPSC line BO-VC1 compared to the HDFa control ( n = 3 different passages) Moreover, generated iPSCs were functionally validated by directed differentiation into all three germ layers. Subsequent immunocytochemical staining of the iPSCs before and after differentiation confirmed the expression of ( J–M ) meso-, N–Q endo- and R–U ectodermal lineage markers only in the respective differentiations. Scale bars: 50 μm. Data were tested for normal distribution using Shapiro-Wilk test. Means ± SEM (standard error of the mean) were statistically analyzed by a Kruskal-Wallis test with Dunn’s multiple comparisons test. n = 3 (* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001)
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    Reprogramming of adult human dermal fibroblasts (HDFa) into induced pluripotent stem cell (iPSC) line BO-VC1. Reprogramming was performed using the Epi5 ™ Episomal iPSC Reprogramming Kit, enabling the generation of ( A ) transgene- and virus-free iPSC line BO-VC1. Successful generation of fibroblast-derived iPSCs was validated by immunocytochemical staining for the pluripotency markers ( B ) SOX2, C TRA 1–60, D OCT4, SSEA4 and E NANOG. Quantification of the generated iPSCs via flow cytometry revealed F 99.31% <t>SSEA4/TRA1-60,</t> G 97.43% SOX2/TRA1-60 and H 98.60% OCT3/4/TRA1-60 positive cells. I Quantification of the transcript levels of the stem cell markers NANOG , OCT4 , REX1 and SOX2 revealed significantly higher mRNA levels in iPSC line BO-VC1 compared to the HDFa control ( n = 3 different passages) Moreover, generated iPSCs were functionally validated by directed differentiation into all three germ layers. Subsequent immunocytochemical staining of the iPSCs before and after differentiation confirmed the expression of ( J–M ) meso-, N–Q endo- and R–U ectodermal lineage markers only in the respective differentiations. Scale bars: 50 μm. Data were tested for normal distribution using Shapiro-Wilk test. Means ± SEM (standard error of the mean) were statistically analyzed by a Kruskal-Wallis test with Dunn’s multiple comparisons test. n = 3 (* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001)
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    Reprogramming of adult human dermal fibroblasts (HDFa) into induced pluripotent stem cell (iPSC) line BO-VC1. Reprogramming was performed using the Epi5 ™ Episomal iPSC Reprogramming Kit, enabling the generation of ( A ) transgene- and virus-free iPSC line BO-VC1. Successful generation of fibroblast-derived iPSCs was validated by immunocytochemical staining for the pluripotency markers ( B ) SOX2, C TRA 1–60, D OCT4, SSEA4 and E NANOG. Quantification of the generated iPSCs via flow cytometry revealed F 99.31% <t>SSEA4/TRA1-60,</t> G 97.43% SOX2/TRA1-60 and H 98.60% OCT3/4/TRA1-60 positive cells. I Quantification of the transcript levels of the stem cell markers NANOG , OCT4 , REX1 and SOX2 revealed significantly higher mRNA levels in iPSC line BO-VC1 compared to the HDFa control ( n = 3 different passages) Moreover, generated iPSCs were functionally validated by directed differentiation into all three germ layers. Subsequent immunocytochemical staining of the iPSCs before and after differentiation confirmed the expression of ( J–M ) meso-, N–Q endo- and R–U ectodermal lineage markers only in the respective differentiations. Scale bars: 50 μm. Data were tested for normal distribution using Shapiro-Wilk test. Means ± SEM (standard error of the mean) were statistically analyzed by a Kruskal-Wallis test with Dunn’s multiple comparisons test. n = 3 (* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001)
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    Reprogramming of adult human dermal fibroblasts (HDFa) into induced pluripotent stem cell (iPSC) line BO-VC1. Reprogramming was performed using the Epi5 ™ Episomal iPSC Reprogramming Kit, enabling the generation of ( A ) transgene- and virus-free iPSC line BO-VC1. Successful generation of fibroblast-derived iPSCs was validated by immunocytochemical staining for the pluripotency markers ( B ) SOX2, C TRA 1–60, D OCT4, SSEA4 and E NANOG. Quantification of the generated iPSCs via flow cytometry revealed F 99.31% <t>SSEA4/TRA1-60,</t> G 97.43% SOX2/TRA1-60 and H 98.60% OCT3/4/TRA1-60 positive cells. I Quantification of the transcript levels of the stem cell markers NANOG , OCT4 , REX1 and SOX2 revealed significantly higher mRNA levels in iPSC line BO-VC1 compared to the HDFa control ( n = 3 different passages) Moreover, generated iPSCs were functionally validated by directed differentiation into all three germ layers. Subsequent immunocytochemical staining of the iPSCs before and after differentiation confirmed the expression of ( J–M ) meso-, N–Q endo- and R–U ectodermal lineage markers only in the respective differentiations. Scale bars: 50 μm. Data were tested for normal distribution using Shapiro-Wilk test. Means ± SEM (standard error of the mean) were statistically analyzed by a Kruskal-Wallis test with Dunn’s multiple comparisons test. n = 3 (* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001)
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    Image Search Results


    Reprogramming of adult human dermal fibroblasts (HDFa) into induced pluripotent stem cell (iPSC) line BO-VC1. Reprogramming was performed using the Epi5 ™ Episomal iPSC Reprogramming Kit, enabling the generation of ( A ) transgene- and virus-free iPSC line BO-VC1. Successful generation of fibroblast-derived iPSCs was validated by immunocytochemical staining for the pluripotency markers ( B ) SOX2, C TRA 1–60, D OCT4, SSEA4 and E NANOG. Quantification of the generated iPSCs via flow cytometry revealed F 99.31% SSEA4/TRA1-60, G 97.43% SOX2/TRA1-60 and H 98.60% OCT3/4/TRA1-60 positive cells. I Quantification of the transcript levels of the stem cell markers NANOG , OCT4 , REX1 and SOX2 revealed significantly higher mRNA levels in iPSC line BO-VC1 compared to the HDFa control ( n = 3 different passages) Moreover, generated iPSCs were functionally validated by directed differentiation into all three germ layers. Subsequent immunocytochemical staining of the iPSCs before and after differentiation confirmed the expression of ( J–M ) meso-, N–Q endo- and R–U ectodermal lineage markers only in the respective differentiations. Scale bars: 50 μm. Data were tested for normal distribution using Shapiro-Wilk test. Means ± SEM (standard error of the mean) were statistically analyzed by a Kruskal-Wallis test with Dunn’s multiple comparisons test. n = 3 (* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001)

    Journal: Stem Cell Research & Therapy

    Article Title: Activation of the G-protein coupled estrogen receptor 1 (GPER1) reduces transient receptor potential vanilloid 1 (TRPV1) activity and human iPSC-derived nociceptive neuron firing

    doi: 10.1186/s13287-026-05174-3

    Figure Lengend Snippet: Reprogramming of adult human dermal fibroblasts (HDFa) into induced pluripotent stem cell (iPSC) line BO-VC1. Reprogramming was performed using the Epi5 ™ Episomal iPSC Reprogramming Kit, enabling the generation of ( A ) transgene- and virus-free iPSC line BO-VC1. Successful generation of fibroblast-derived iPSCs was validated by immunocytochemical staining for the pluripotency markers ( B ) SOX2, C TRA 1–60, D OCT4, SSEA4 and E NANOG. Quantification of the generated iPSCs via flow cytometry revealed F 99.31% SSEA4/TRA1-60, G 97.43% SOX2/TRA1-60 and H 98.60% OCT3/4/TRA1-60 positive cells. I Quantification of the transcript levels of the stem cell markers NANOG , OCT4 , REX1 and SOX2 revealed significantly higher mRNA levels in iPSC line BO-VC1 compared to the HDFa control ( n = 3 different passages) Moreover, generated iPSCs were functionally validated by directed differentiation into all three germ layers. Subsequent immunocytochemical staining of the iPSCs before and after differentiation confirmed the expression of ( J–M ) meso-, N–Q endo- and R–U ectodermal lineage markers only in the respective differentiations. Scale bars: 50 μm. Data were tested for normal distribution using Shapiro-Wilk test. Means ± SEM (standard error of the mean) were statistically analyzed by a Kruskal-Wallis test with Dunn’s multiple comparisons test. n = 3 (* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001)

    Article Snippet: After harvesting, using Accutase, 1 × 10 6 cells were stained for the surface stem cell marker TRA1-60 (1:50, #130-122-965, Miltenyi Biotec, Bergisch Gladbach, Germany) and SSEA4 (1:50, #130-124-073, Miltenyi Biotec, Bergisch Gladbach, Germany) in 25 μl PEB buffer (PBS + 0.5% BSA) for 10 min at 4 °C, washed in 500 μl PEB buffer and centrifuged at 200xg for 5 min.

    Techniques: Virus, Derivative Assay, Staining, Generated, Flow Cytometry, Control, Expressing